Product

Protein Isolation

PhosPRO™ Phosphoprotein Purification Kit
New Version of IMAC Based Purification Reagent.

PhosPRO™ provides a new version of IMAC based purification method and delivers excellent purification result. PhosPRO™ can specifically purify the phosphoprotein from the protein sample by simple process with our proprietary chromatographic technology. PhosPRO™ can also successfully purify phosphoprotein from different biological materials, such as animal, plant, and microorganism, and is suitable for applying quality required analysis.
Product information

Highlights:

 

  • More flexible: PhosPRO™ provides stringent buffer stock for the customer to optimize washing conditions for their sample and to maximize the target phosphoprotein purification result.
  • More choices: PhosPRO™ series have 2 versions with 2 different packages for different purposes

     (1) The pre-packed column package is designed for proteomics research and can purify phosphoprotein within 1 hour. The purified sample can directly be analyzed by two-dimensional electrophoresis or mass spectrometry.

     (2) Evaluation package is designed for phosphoproteomics research and researcher can small scale optimizes suitable chromatographic conditions for the target phosphoprotein.

 

 

Order Information:

 

Cat. No. Product Name Description
PP05-2C  PhosPRO™ Phosphoprotein Purification Kit

  Pre-packed column package

  2 pre-packed column + 2ml Lysis buffer + 250ml System buffer + 100ml Elution buffer + 100ml stringent buffer stock

PP05-6C PhosPRO™ Phosphoprotein Purification Kit

  Pre-packed column package

  6 pre-packed column + 2ml Lysis buffer + 250ml System buffer + 100ml Elution buffer + 100ml stringent buffer stock

PP05-5E PhosPRO™ Phosphoprotein Purification Kit

  Evaluation package 3ml resin + 2ml Lysis buffer + 250ml System buffer + 100ml Elution buffer + 100ml stringent buffer stock

 

 

 

Product Detail:

 

 

Figure 1. Purification efficacy of PhosPRO™ Phosphoprotein Purification Kit

(A) and Brand Q kit (B). Protein standard markers contain glycogen phosphorylase b (GP), bovine serum albumin (BSA), ovalbumin (OVA), carbonic anhydrase (CA), trypsin inhibitor (TI), and lactalbumin (LAC) were used for evaluation. Ovalbumin was the only phosphoprotein in the test material. F: flow through; E: eluent; VisPRO™ 5 minute Protein Stain Kit (VisPRO™) and Pro-Q Diamond stain (Pro-Q, from Invitrogen) were used for developing total proteins or phosphoproteins.

 

 

 

 

Figure 2. Optimize suitable chromatographic conditions with PhosPRO™ Phosphoprotein Purification Kit.