Western Blotting

LumiFlash™ Femto Chemiluminescent substrate, HRP System
LumiFlash™ Femto Chemiluminescent Substrate is the most sensitive chemiluminescent reagent in our LumiFlash™ Series products. It is an extremely sensitive ECL (enhanced chemiluminescent substrate) for detection of low-femtogram level protein with horseradish peroxidase (HRP) in Western blotting. LumiFlash™ Femto provides high intense signal output resulting in a brighter background on both PVDF and nitrocellulose membrane. It fulfills the need for detection of target proteins in trace amounts which are too small to be detected with general ECL substrate.
Product information



  • Very High signal sensitivity: detection of target protein at the low-femtogram level
  • Long duration: signal duration up to 12 hours
  • Long shelf life: 18 months long at 4℃



Order Information:


Cat. No. Product Name Description
LF24-100 LumiFlash™ Femto Chemiluminescent Substrate, HRP System

50mL Solution A + 50mL Solution B




Product Detail:



Figure 1. Western blotting image of LumiFlash™ Femto Chemiluminescent Substrate, HRP System.

Hela cell lysate with 1/2 serial dilution from 7 µg was separated by 12.5% SDS-PAGE. The proteins were transferred to PVDF and blocked for 1 min at room temperature with BlockPRO™ 1 Min (#BM01-500). (A) The blot was probed at 1:2,500 with Anti-Akt antibody (#4691, Cell Signaling Technology). (B) The blot was probed at 1:10,000 with Anti-GAPDH antibody (#ab8245, Abcam). A HRP-conjugated secondary antibody was applied and developed with LumiFlash™ Femto Chemiluminescent Substrate (#LF24-100). All blots were simultaneously exposed for 10 seconds, 30 seconds, and 60 seconds using ChemLux SPX-600 imaging system.



LumiFlash™ Series:






1. Huang, Yen-Wen, et al. "2, 3, 5, 4′-tetrahydroxystilbene-2-ObD-glucoside triggers the pluripotent-like possibility of dental pulp stem cells by activating the JAK2/STAT3 axis: Preliminary observations." Journal of Dental Sciences (2020). Link

2. Mou D-F, Chen W-T, Li W-H, Chen T-C, Tseng C-H, Huang L-H, et al. (2021) Transmission mode of watermelon silver mottle virus by Thrips palmi. PLoS ONE 16(3): e0247500. Link

3. Lin, Yuh-Charn, et al. "SCUBE3 loss-of-function causes a recognizable recessive developmental disorder due to defective bone morphogenetic protein signaling." The American Journal of Human Genetics 108.1 (2021): 115-133. Link