Western Blotting

LuminolPen™, HRP system
Find your disappear molecular weight.

The LuminolPen™ HRP System is a chemiluminescent marker pen designed for precise data management in Western blotting. Visible blue ink reacts with ECL substrates, allowing annotations directly on PVDF or NC membranes. Use it to mark pre-stained protein positions, experiment conditions, and operation dates before blocking. Simplify your process with clear, effective documentation.
Product information



  • Unique: the world's first luminescent color development marker pen
  • Help you to evaluate the efficacy of your ECL substrate
  • Data manegement: able to make notes and show on the image with the data result
  • Compatible: applicable with most brands of ECL substrate



Order Information:


Cat. No. Product Name Description
LH03-50 LuminolPen™, HRP System

About 1,000 membranes drawing

LH03-10 LuminolPen™, HRP System

About 100 membranes drawing




Product Detail :



Figure 1. Labeling molecular weight and note on the membrane with LuminolPen™.

 (A) and (C) are general Western blot chemiluminescence detection image; (B) and (D) are the image using LuminolPen™ to indicate the pre-stained marker.





Figure 2. Distinguish target signal and non-specific binding signal with LuminolPen™

30 μg cell lysate SP-1 and detect with anti-ADNP (mouse, 1:2,000). Secondary antibody : anti-mouse IgG-HRP (1:15,000). Membrane : Hyond™ P. Detection : Hyperfilm™ ECL. Signal exposed for 30 seconds and detected by X-ray. The molecular weight note by LuminolPen™ reveal the correct target signal (ADNP 124KDa) on X-ray film.





Figure 3. Evaluate ECL substrate efficacy by LuminolPen™.

30 μg cell lysate HepaG2 and detect with anti-AMP-active protein kinase (mouse, 1:1,000). Secondary antibody : anti-mouse IgG-HRP (1:10,000). Membrane: Hyond™ P. Detection: Hyperfilm™ ECL. Signal exposed for 30 seconds and detected by X-ray. The signal result present by the normal ECL substrate is stronger than using the expired ECL substrate. LuminolPen™ noted marker can be an indicator to check the result or adjust different experiments to similar experimental conditions.



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