Western Blotting

BlockPRO™ 1 Min Protein-Free Blocking Buffer
Complete the blocking step in only 1 minute.

BlockPRO™ 1 Min Protein-Free Blocking Buffer is a fast blocking buffer for Western blotting with non-protein formulation containing 0.05% Tween-20 surfactant. It effectively prevents protein cross-reaction and improves the signal/noise ratio of the detected signals by one-minute blocking. BlockPRO™ 1 Min Protein-Free Blocking Buffer can be applied to immunodetection systems including antibodies and biotin/avidin systems such as ELISA, Western blotting, immunohistochemistry and other immunochemical applications.



  • Fast blocking: the enhanced formula shortens the blocking time to 1 minute
  • Protein-free: eliminates cross-reactivity happened in traditional protein-based blocking buffers
  • Antibody diluent: for diluting primary and secondary antibodies and extending the storage time of antibodies



Order Information:

Cat. No. Product Name Description

BlockPRO™ 1 Min Protein-Free Blocking Buffer 

500mL Solution X 1 

BM10-100 BlockPRO™ 1 Min Protein-Free Blocking Buffer (10X)

100mL Solution X 1




Product Detail:




Figure 1. BlockPRO™ 1 Min Protein-Free Blocking Buffer enhanced the signal intensity and shorten the blocking time to 1 min. 

20 μg of Huh-7 cell lysates were serial diluted and separated by electrophoresis. The proteins were transferred to PVDF and blocked for 1 min hour at room temperature with BlockPRO™ 1 Min or 1 hour in competitor's product (as indicated), probed with mouse anti-ACC followed by anti-mouse HRP and detected with LumiFlash™ Ultima Chemiluminescent Substrate.



Selection Guide:




1. Hsueh, Cheng-Shun, et al. "Role of nuclear factor-kappa B in feline injection site sarcoma." BMC veterinary research 15.1 (2019): 365. (WB)

2. Lin, Yuan‐Hau, et al. “The study of isotopic enrichment of water in human plasma and erythrocyte.” The FASEB Journal 34.9 (2020): 13049-13062. (WB)

3. Su, Kuan-Wei, et al. “Repurposing cabozantinib with therapeutic potential in KIT-driven t (8; 21) acute myeloid leukaemias.” Cancer Gene Therapy (2021): 1-14. (WB)

4. Chen, Shih-Heng, et al. "Extracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons." International Journal of Molecular Sciences 22.22 (2021): 12373. (WB)

5. Shih, Cheng-Hsin, et al. "Investigating the role of signal transducer and activator of transcription 3 in feline injection site sarcoma." BMC Veterinary Research 18.1 (2022): 1-15. (IHC)

6. Wang, Ya‐Chin, et al. "NPRL2 down‐regulation facilitates the growth of hepatocellular carcinoma via the mTOR pathway and autophagy suppression." Hepatology Communications 6.12 (2022): 3563-3577. (WB)

7. Chang, Hsin-Han, et al. "ONX-0914 Induces Apoptosis and Autophagy with p53 Regulation in Human Glioblastoma Cells." Cancers 14.22 (2022): 5712. (WB)

8. Tang, Ya-Xin, et al. "Lipophilic constituents in Salvia miltiorrhiza Inhibit activation of the hepatic stellate cells by suppressing the JAK1/STAT3 signaling pathway: A network pharmacology study and experimental validation." Frontiers in Pharmacology 13 (2022): 770344. (WB)

9. Hsiao, Wen-Wei, et al. "Antifungal Activity of Cedrol from Cunninghamia lanceolate var. konishii against Phellinus noxius and Its Mechanism." Plants 13.2 (2024): 321. (WB)

10. Chen, Shih-Heng, et al. "The Transplantation of 3-Dimensional Spheroids of Adipose-Derived Stem Cells Promotes Achilles Tendon Healing in Rabbits by Enhancing the Proliferation of Tenocytes and Suppressing M1 Macrophages." The American Journal of Sports Medicine (2024): 03635465231214698. (WB)

11. Cheng, Chia-Hsiung, et al. "Plasmon-activated water as a therapeutic strategy in Alzheimer’s disease by altering gut microbiota." Aging (Albany NY) 15.9 (2023): 3715. (WB)

12. Chomthong, Kittima, et al. "Label-free simultaneous detection of quinolone antibiotic residues using an origami paper–based electrochemical immunosensor." Sensors and Actuators B: Chemical 410 (2024): 135667. (immunosensor)