LuminolPen™, HRP System

 

Find your disappear molecular weight

LuminolPen™, HRP System is a perfect tool for chemiluminescent detection in Western blot. With simply writing on the pre-stained marker, the position of the molecular weight can be showed on the image. It could also help you to note the experiment condition or evaluate the efficacy of ECL substrate.

 

 

  Special Characteristic

  • Unique : the world’s first luminescent color development marker pen
  • Help you to evaluate the efficacy of your ECL substrate
  • Convenient : able to make note and show on the image with the data result
  • Compatible : applicable with most brands of ECL substrate  

 

  Order Information

Cat. No. Product Name Description

 

LH03-50

 

  LuminolPen™, HRP System

 

  About 1,000 membranes drawing

 

 

LH03-10

 

  LuminolPen™, HRP System

 

  About 100 membranes drawing

 

 

 

 

 

 

 

  Product Detail

 

Figure 1. Labeling molecular weight and note on the membrane with LuminolPen™. (A) and (C) are general Western blot chemiluminescence detection image; (B) and (D) are the image using LuminolPen™ to indicate the pre-stained marker.

 

 

Figure 2. Distinguish target signal and non-specific binding signal with LuminolPen™. 30 μg cell lysate SP-1 and detect with anti-ADNP (mouse, 1:2,000). Secondary antibody : anti-mouse IgG-HRP (1:15,000). Membrane : Hyond™ P. Detection : Hyperfilm™ ECL. Signal exposed for 30 seconds and detected by X-ray. The molecular weight note by LuminolPen™ reveal the correct target signal (ADNP 124KDa) on X-ray film.

 

 

Figure 3. Evaluate ECL substrate efficacy by LuminolPen™. 30 μg cell lysate HepaG2 and detect with anti-AMP-active protein kinase (mouse, 1:1,000). Secondary antibody : anti-mouse IgG-HRP (1:10,000). Membrane : Hyond™ P. Detection : Hyperfilm™ ECL. Signal exposed for 30 seconds and detected by X-ray. Signal result present by normal ECL substrate is stronger than using expired ECL substrate. LuminolPen™ noted marker can be an indicator to check the result or adjust different experiment to similar experimental condition.

 

 

 
 

 

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