LumiFlash™ Infinity Chemiluminescent Substrate, HRP


The most sensitive ECL substrate for Western blotting analysis

LumiFlash™ Infinity Chemiluminescent Substrate, HRP is an extremely sensitive enhanced chemiluminescent substrate for femtogram-level by detection of immobilized proteins (Western blotting). In Western blotting or ELISA application, LumiFlash™ Infinity Chemiluminescent Substrate, HRP provides high signal and low background, which allows detection of target protein on PVDF or nitrocellulose. This feature benefits the researchers with excellent low background result and without signal burn effect at the same time.



    Special Characteristic

  • High sensitivity – detect femtogram of protein target on PVDF or nitrocellulose membrane
  • Antibody saving – use very low antibody concentration on the WB and ELISA application; dilute
                                    primary antibody 5000 to 20,000-fold and secondary antibody 100,000 to 400,000-fold
                                    (from 1mg/mL stock)
  • Low background – provide insignificant background and avoid non-specific signals on the WB result.   


  Order Information

Cat. No. Product Name Description




  LumiFlash™ Infinity Chemiluminescent Substrate, HRP



  250mL soultion A + 250mL solution B





  Product Detail



Figure 1: The comparison of WB application for LumiFlash Infinity and Luminata Forte. Hela cell lysate with 1/2 serial dilution from 20 µg was separated by 12.5% SDS-PAGE and probed by anti AMPKa1. All results were exposed to X-ray film for 5 minutes.